Cross-species Cell Atlas (CSCA) provides single-cell transcriptomic profiles across 14 reproductive and CNS tissues from sheep and humans. By mapping cell-type-specific gene expression programs, the atlas reveals conserved and divergent regulatory mechanisms underlying reproductive development and traits. This resource serves as a comparative platform to advance understanding of mammalian reproduction and supports the use of sheep as a model for human reproductive biology.
We generated a comprehensive single-cell transcriptomic dataset spanning 15 key reproductive and central nervous system (CNS) tissues in sheep, including the cerebral cortex, hippocampus, hypothalamus, pineal gland, pituitary, mammary gland, ovary, oviduct, placenta, uterus, prostate, testis, vas deferens, and skeletal muscle. In parallel, we integrated publicly available human single-cell RNA-seq data covering 13 matched tissues (excluding prostate and epididymis). The sheep dataset, produced from our own experimental sequencing, comprises a total of 316,616 high-quality cells (accession code:XXX), while the human dataset contains 769,901 high-quality cells. Together, these cross-species single-cell profiles provide a valuable resource for understanding the conserved and divergent cellular landscapes and transcriptional programs across reproductive and CNS systems between sheep and humans.
Bingru Zhao zhaobingru@njau.edu.cn
Yanli Zhang zhangyanli@njau.edu.cn
Jiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China
Biodiversity Data Center kizdatacenter@mail.kiz.ac.cn
Biodiversity Data Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan, Kunming, 650201, China